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antibody against chlamydia momp  (Novus Biologicals)


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    Structured Review

    Novus Biologicals antibody against chlamydia momp
    Antibody Against Chlamydia Momp, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibody+against+chlamydia+momp/Chlamydia+Momp+Antibody+-+BSA+Free/pmc12694302-124-15-20
    Average 93 stars, based on 8 article reviews
    antibody against chlamydia momp - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Infection:

    Article Title: Chlamydia muridarum Infection Impacts Murine Models of Intestinal Inflammation and Cancer
    Article Snippet: 1 Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX; Leica Biosystems, Buffalo Grove, IL).1 Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX; Leica Biosystems, Buffalo Grove, IL).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054; Novus Biologicals, Centennial, CO) was applied at a dilution of 1:2,000.. A rabbit anti-goat secondary antibody (cat. no. BA-5000; Vector Laboratories, Newark, CA) and a polymer detection system (DS9800; Novocastra Bond Polymer Refine Detection; Leica Biosystems, Buffalo Grove, IL) were then applied to the tissues.A rabbit anti-goat secondary antibody (cat. no. BA-5000; Vector Laboratories, Newark, CA) and a polymer detection system (DS9800; Novocastra Bond Polymer Refine Detection; Leica Biosystems, Buffalo Grove, IL) were then applied to the tissues.

    Article Title: Chlamydia muridarum Infection Impacts Murine Models of Intestinal Inflammation and Cancer
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems, Deer Park, IL).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems, Deer Park, IL).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:2000.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Assessing the Biosecurity Risk of Footwear as a Fomite for Transmission of Adventitious Infectious Agents to Mice
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (catalog no. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra bond polymer refine detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (catalog no. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra bond polymer refine detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Assessing the Biosecurity Risk of Footwear as a Fomite for Transmission of Adventitious Infectious Agents to Mice
    Article Snippet: Briefly, formalin-fixed paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Chlamydia muridarum infection causes bronchointerstitial pneumonia in NOD.Cg- Prkdc scid Il2rg tm1Wjl /SzJ (NSG) mice
    Article Snippet: Formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, Colorado) was applied at a dilution of 1:2000.. A rabbit antigoat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, California) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit antigoat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, California) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Chlamydia muridarum Causes Persistent Subclinical Infection and Elicits Innate and Adaptive Immune Responses in C57BL/6J, BALB/cJ and J:ARC(S) Mice Following Exposure to Shedding Mice
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    H&E Stain:

    Article Title: Chlamydia muridarum Infection Impacts Murine Models of Intestinal Inflammation and Cancer
    Article Snippet: 1 Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX; Leica Biosystems, Buffalo Grove, IL).1 Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX; Leica Biosystems, Buffalo Grove, IL).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054; Novus Biologicals, Centennial, CO) was applied at a dilution of 1:2,000.. A rabbit anti-goat secondary antibody (cat. no. BA-5000; Vector Laboratories, Newark, CA) and a polymer detection system (DS9800; Novocastra Bond Polymer Refine Detection; Leica Biosystems, Buffalo Grove, IL) were then applied to the tissues.A rabbit anti-goat secondary antibody (cat. no. BA-5000; Vector Laboratories, Newark, CA) and a polymer detection system (DS9800; Novocastra Bond Polymer Refine Detection; Leica Biosystems, Buffalo Grove, IL) were then applied to the tissues.

    Article Title: Chlamydia muridarum Infection Impacts Murine Models of Intestinal Inflammation and Cancer
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems, Deer Park, IL).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems, Deer Park, IL).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:2000.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Assessing the Biosecurity Risk of Footwear as a Fomite for Transmission of Adventitious Infectious Agents to Mice
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (catalog no. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra bond polymer refine detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (catalog no. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra bond polymer refine detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Assessing the Biosecurity Risk of Footwear as a Fomite for Transmission of Adventitious Infectious Agents to Mice
    Article Snippet: Briefly, formalin-fixed paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Chlamydia muridarum infection causes bronchointerstitial pneumonia in NOD.Cg- Prkdc scid Il2rg tm1Wjl /SzJ (NSG) mice
    Article Snippet: Formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, Colorado) was applied at a dilution of 1:2000.. A rabbit antigoat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, California) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit antigoat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, California) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Chlamydia muridarum Causes Persistent Subclinical Infection and Elicits Innate and Adaptive Immune Responses in C57BL/6J, BALB/cJ and J:ARC(S) Mice Following Exposure to Shedding Mice
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Control:

    Article Title: Chlamydia muridarum Infection Impacts Murine Models of Intestinal Inflammation and Cancer
    Article Snippet: 1 Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX; Leica Biosystems, Buffalo Grove, IL).1 Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX; Leica Biosystems, Buffalo Grove, IL).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054; Novus Biologicals, Centennial, CO) was applied at a dilution of 1:2,000.. A rabbit anti-goat secondary antibody (cat. no. BA-5000; Vector Laboratories, Newark, CA) and a polymer detection system (DS9800; Novocastra Bond Polymer Refine Detection; Leica Biosystems, Buffalo Grove, IL) were then applied to the tissues.A rabbit anti-goat secondary antibody (cat. no. BA-5000; Vector Laboratories, Newark, CA) and a polymer detection system (DS9800; Novocastra Bond Polymer Refine Detection; Leica Biosystems, Buffalo Grove, IL) were then applied to the tissues.

    Article Title: Chlamydia muridarum Infection Impacts Murine Models of Intestinal Inflammation and Cancer
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems, Deer Park, IL).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems, Deer Park, IL).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:2000.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Assessing the Biosecurity Risk of Footwear as a Fomite for Transmission of Adventitious Infectious Agents to Mice
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (catalog no. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra bond polymer refine detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (catalog no. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra bond polymer refine detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Assessing the Biosecurity Risk of Footwear as a Fomite for Transmission of Adventitious Infectious Agents to Mice
    Article Snippet: Briefly, formalin-fixed paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Chlamydia muridarum infection causes bronchointerstitial pneumonia in NOD.Cg- Prkdc scid Il2rg tm1Wjl /SzJ (NSG) mice
    Article Snippet: Formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, Colorado) was applied at a dilution of 1:2000.. A rabbit antigoat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, California) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit antigoat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, California) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Chlamydia muridarum Causes Persistent Subclinical Infection and Elicits Innate and Adaptive Immune Responses in C57BL/6J, BALB/cJ and J:ARC(S) Mice Following Exposure to Shedding Mice
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Isolation:

    Article Title: Chlamydia muridarum Infection Impacts Murine Models of Intestinal Inflammation and Cancer
    Article Snippet: 1 Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX; Leica Biosystems, Buffalo Grove, IL).1 Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX; Leica Biosystems, Buffalo Grove, IL).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054; Novus Biologicals, Centennial, CO) was applied at a dilution of 1:2,000.. A rabbit anti-goat secondary antibody (cat. no. BA-5000; Vector Laboratories, Newark, CA) and a polymer detection system (DS9800; Novocastra Bond Polymer Refine Detection; Leica Biosystems, Buffalo Grove, IL) were then applied to the tissues.A rabbit anti-goat secondary antibody (cat. no. BA-5000; Vector Laboratories, Newark, CA) and a polymer detection system (DS9800; Novocastra Bond Polymer Refine Detection; Leica Biosystems, Buffalo Grove, IL) were then applied to the tissues.

    Article Title: Chlamydia muridarum Infection Impacts Murine Models of Intestinal Inflammation and Cancer
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems, Deer Park, IL).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems, Deer Park, IL).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:2000.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Assessing the Biosecurity Risk of Footwear as a Fomite for Transmission of Adventitious Infectious Agents to Mice
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (catalog no. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra bond polymer refine detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (catalog no. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra bond polymer refine detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Assessing the Biosecurity Risk of Footwear as a Fomite for Transmission of Adventitious Infectious Agents to Mice
    Article Snippet: Briefly, formalin-fixed paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Chlamydia muridarum infection causes bronchointerstitial pneumonia in NOD.Cg- Prkdc scid Il2rg tm1Wjl /SzJ (NSG) mice
    Article Snippet: Formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, Colorado) was applied at a dilution of 1:2000.. A rabbit antigoat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, California) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit antigoat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, California) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Chlamydia muridarum Causes Persistent Subclinical Infection and Elicits Innate and Adaptive Immune Responses in C57BL/6J, BALB/cJ and J:ARC(S) Mice Following Exposure to Shedding Mice
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Immunohistochemistry:

    Article Title: Chlamydia muridarum Infection Impacts Murine Models of Intestinal Inflammation and Cancer
    Article Snippet: 1 Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX; Leica Biosystems, Buffalo Grove, IL).1 Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX; Leica Biosystems, Buffalo Grove, IL).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054; Novus Biologicals, Centennial, CO) was applied at a dilution of 1:2,000.. A rabbit anti-goat secondary antibody (cat. no. BA-5000; Vector Laboratories, Newark, CA) and a polymer detection system (DS9800; Novocastra Bond Polymer Refine Detection; Leica Biosystems, Buffalo Grove, IL) were then applied to the tissues.A rabbit anti-goat secondary antibody (cat. no. BA-5000; Vector Laboratories, Newark, CA) and a polymer detection system (DS9800; Novocastra Bond Polymer Refine Detection; Leica Biosystems, Buffalo Grove, IL) were then applied to the tissues.

    Article Title: Chlamydia muridarum Infection Impacts Murine Models of Intestinal Inflammation and Cancer
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems, Deer Park, IL).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems, Deer Park, IL).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:2000.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Assessing the Biosecurity Risk of Footwear as a Fomite for Transmission of Adventitious Infectious Agents to Mice
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (catalog no. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra bond polymer refine detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (catalog no. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra bond polymer refine detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Assessing the Biosecurity Risk of Footwear as a Fomite for Transmission of Adventitious Infectious Agents to Mice
    Article Snippet: Briefly, formalin-fixed paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Chlamydia muridarum infection causes bronchointerstitial pneumonia in NOD.Cg- Prkdc scid Il2rg tm1Wjl /SzJ (NSG) mice
    Article Snippet: Formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, Colorado) was applied at a dilution of 1:2000.. A rabbit antigoat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, California) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit antigoat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, California) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Chlamydia muridarum Causes Persistent Subclinical Infection and Elicits Innate and Adaptive Immune Responses in C57BL/6J, BALB/cJ and J:ARC(S) Mice Following Exposure to Shedding Mice
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Membrane:

    Article Title: Chlamydia muridarum Infection Impacts Murine Models of Intestinal Inflammation and Cancer
    Article Snippet: 1 Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX; Leica Biosystems, Buffalo Grove, IL).1 Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX; Leica Biosystems, Buffalo Grove, IL).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054; Novus Biologicals, Centennial, CO) was applied at a dilution of 1:2,000.. A rabbit anti-goat secondary antibody (cat. no. BA-5000; Vector Laboratories, Newark, CA) and a polymer detection system (DS9800; Novocastra Bond Polymer Refine Detection; Leica Biosystems, Buffalo Grove, IL) were then applied to the tissues.A rabbit anti-goat secondary antibody (cat. no. BA-5000; Vector Laboratories, Newark, CA) and a polymer detection system (DS9800; Novocastra Bond Polymer Refine Detection; Leica Biosystems, Buffalo Grove, IL) were then applied to the tissues.

    Article Title: Chlamydia muridarum Infection Impacts Murine Models of Intestinal Inflammation and Cancer
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems, Deer Park, IL).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems, Deer Park, IL).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:2000.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Assessing the Biosecurity Risk of Footwear as a Fomite for Transmission of Adventitious Infectious Agents to Mice
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (catalog no. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra bond polymer refine detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (catalog no. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra bond polymer refine detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Assessing the Biosecurity Risk of Footwear as a Fomite for Transmission of Adventitious Infectious Agents to Mice
    Article Snippet: Briefly, formalin-fixed paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Chlamydia muridarum infection causes bronchointerstitial pneumonia in NOD.Cg- Prkdc scid Il2rg tm1Wjl /SzJ (NSG) mice
    Article Snippet: Formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, Colorado) was applied at a dilution of 1:2000.. A rabbit antigoat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, California) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit antigoat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, California) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Chlamydia muridarum Causes Persistent Subclinical Infection and Elicits Innate and Adaptive Immune Responses in C57BL/6J, BALB/cJ and J:ARC(S) Mice Following Exposure to Shedding Mice
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    In Situ Hybridization:

    Article Title: Chlamydia muridarum Infection Impacts Murine Models of Intestinal Inflammation and Cancer
    Article Snippet: 1 Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX; Leica Biosystems, Buffalo Grove, IL).1 Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX; Leica Biosystems, Buffalo Grove, IL).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054; Novus Biologicals, Centennial, CO) was applied at a dilution of 1:2,000.. A rabbit anti-goat secondary antibody (cat. no. BA-5000; Vector Laboratories, Newark, CA) and a polymer detection system (DS9800; Novocastra Bond Polymer Refine Detection; Leica Biosystems, Buffalo Grove, IL) were then applied to the tissues.A rabbit anti-goat secondary antibody (cat. no. BA-5000; Vector Laboratories, Newark, CA) and a polymer detection system (DS9800; Novocastra Bond Polymer Refine Detection; Leica Biosystems, Buffalo Grove, IL) were then applied to the tissues.

    Article Title: Chlamydia muridarum Infection Impacts Murine Models of Intestinal Inflammation and Cancer
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems, Deer Park, IL).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems, Deer Park, IL).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:2000.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Assessing the Biosecurity Risk of Footwear as a Fomite for Transmission of Adventitious Infectious Agents to Mice
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (catalog no. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra bond polymer refine detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (catalog no. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra bond polymer refine detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Assessing the Biosecurity Risk of Footwear as a Fomite for Transmission of Adventitious Infectious Agents to Mice
    Article Snippet: Briefly, formalin-fixed paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Chlamydia muridarum infection causes bronchointerstitial pneumonia in NOD.Cg- Prkdc scid Il2rg tm1Wjl /SzJ (NSG) mice
    Article Snippet: Formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, Colorado) was applied at a dilution of 1:2000.. A rabbit antigoat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, California) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit antigoat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, California) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Chlamydia muridarum Causes Persistent Subclinical Infection and Elicits Innate and Adaptive Immune Responses in C57BL/6J, BALB/cJ and J:ARC(S) Mice Following Exposure to Shedding Mice
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Immunolabeling:

    Article Title: Chlamydia muridarum Infection Impacts Murine Models of Intestinal Inflammation and Cancer
    Article Snippet: 1 Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX; Leica Biosystems, Buffalo Grove, IL).1 Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX; Leica Biosystems, Buffalo Grove, IL).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054; Novus Biologicals, Centennial, CO) was applied at a dilution of 1:2,000.. A rabbit anti-goat secondary antibody (cat. no. BA-5000; Vector Laboratories, Newark, CA) and a polymer detection system (DS9800; Novocastra Bond Polymer Refine Detection; Leica Biosystems, Buffalo Grove, IL) were then applied to the tissues.A rabbit anti-goat secondary antibody (cat. no. BA-5000; Vector Laboratories, Newark, CA) and a polymer detection system (DS9800; Novocastra Bond Polymer Refine Detection; Leica Biosystems, Buffalo Grove, IL) were then applied to the tissues.

    Article Title: Chlamydia muridarum Infection Impacts Murine Models of Intestinal Inflammation and Cancer
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems, Deer Park, IL).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems, Deer Park, IL).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:2000.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Assessing the Biosecurity Risk of Footwear as a Fomite for Transmission of Adventitious Infectious Agents to Mice
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (catalog no. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra bond polymer refine detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (catalog no. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra bond polymer refine detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Assessing the Biosecurity Risk of Footwear as a Fomite for Transmission of Adventitious Infectious Agents to Mice
    Article Snippet: Briefly, formalin-fixed paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Chlamydia muridarum infection causes bronchointerstitial pneumonia in NOD.Cg- Prkdc scid Il2rg tm1Wjl /SzJ (NSG) mice
    Article Snippet: Formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, Colorado) was applied at a dilution of 1:2000.. A rabbit antigoat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, California) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit antigoat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, California) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Chlamydia muridarum Causes Persistent Subclinical Infection and Elicits Innate and Adaptive Immune Responses in C57BL/6J, BALB/cJ and J:ARC(S) Mice Following Exposure to Shedding Mice
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Immunofluorescence:

    Article Title: Chlamydia muridarum Infection Impacts Murine Models of Intestinal Inflammation and Cancer
    Article Snippet: 1 Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX; Leica Biosystems, Buffalo Grove, IL).1 Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX; Leica Biosystems, Buffalo Grove, IL).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054; Novus Biologicals, Centennial, CO) was applied at a dilution of 1:2,000.. A rabbit anti-goat secondary antibody (cat. no. BA-5000; Vector Laboratories, Newark, CA) and a polymer detection system (DS9800; Novocastra Bond Polymer Refine Detection; Leica Biosystems, Buffalo Grove, IL) were then applied to the tissues.A rabbit anti-goat secondary antibody (cat. no. BA-5000; Vector Laboratories, Newark, CA) and a polymer detection system (DS9800; Novocastra Bond Polymer Refine Detection; Leica Biosystems, Buffalo Grove, IL) were then applied to the tissues.

    Article Title: Chlamydia muridarum Infection Impacts Murine Models of Intestinal Inflammation and Cancer
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems, Deer Park, IL).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems, Deer Park, IL).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:2000.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Assessing the Biosecurity Risk of Footwear as a Fomite for Transmission of Adventitious Infectious Agents to Mice
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (catalog no. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra bond polymer refine detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (catalog no. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra bond polymer refine detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Assessing the Biosecurity Risk of Footwear as a Fomite for Transmission of Adventitious Infectious Agents to Mice
    Article Snippet: Briefly, formalin-fixed paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Chlamydia muridarum infection causes bronchointerstitial pneumonia in NOD.Cg- Prkdc scid Il2rg tm1Wjl /SzJ (NSG) mice
    Article Snippet: Formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, Colorado) was applied at a dilution of 1:2000.. A rabbit antigoat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, California) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit antigoat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, California) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Chlamydia muridarum Causes Persistent Subclinical Infection and Elicits Innate and Adaptive Immune Responses in C57BL/6J, BALB/cJ and J:ARC(S) Mice Following Exposure to Shedding Mice
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Transmission Assay:

    Article Title: Chlamydia muridarum Infection Impacts Murine Models of Intestinal Inflammation and Cancer
    Article Snippet: 1 Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX; Leica Biosystems, Buffalo Grove, IL).1 Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX; Leica Biosystems, Buffalo Grove, IL).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054; Novus Biologicals, Centennial, CO) was applied at a dilution of 1:2,000.. A rabbit anti-goat secondary antibody (cat. no. BA-5000; Vector Laboratories, Newark, CA) and a polymer detection system (DS9800; Novocastra Bond Polymer Refine Detection; Leica Biosystems, Buffalo Grove, IL) were then applied to the tissues.A rabbit anti-goat secondary antibody (cat. no. BA-5000; Vector Laboratories, Newark, CA) and a polymer detection system (DS9800; Novocastra Bond Polymer Refine Detection; Leica Biosystems, Buffalo Grove, IL) were then applied to the tissues.

    Article Title: Chlamydia muridarum Infection Impacts Murine Models of Intestinal Inflammation and Cancer
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems, Deer Park, IL).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems, Deer Park, IL).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:2000.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Assessing the Biosecurity Risk of Footwear as a Fomite for Transmission of Adventitious Infectious Agents to Mice
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (catalog no. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra bond polymer refine detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (catalog no. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra bond polymer refine detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Assessing the Biosecurity Risk of Footwear as a Fomite for Transmission of Adventitious Infectious Agents to Mice
    Article Snippet: Briefly, formalin-fixed paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Chlamydia muridarum infection causes bronchointerstitial pneumonia in NOD.Cg- Prkdc scid Il2rg tm1Wjl /SzJ (NSG) mice
    Article Snippet: Formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, Colorado) was applied at a dilution of 1:2000.. A rabbit antigoat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, California) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit antigoat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, California) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Chlamydia muridarum Causes Persistent Subclinical Infection and Elicits Innate and Adaptive Immune Responses in C57BL/6J, BALB/cJ and J:ARC(S) Mice Following Exposure to Shedding Mice
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Electron Microscopy:

    Article Title: Chlamydia muridarum Infection Impacts Murine Models of Intestinal Inflammation and Cancer
    Article Snippet: 1 Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX; Leica Biosystems, Buffalo Grove, IL).1 Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX; Leica Biosystems, Buffalo Grove, IL).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054; Novus Biologicals, Centennial, CO) was applied at a dilution of 1:2,000.. A rabbit anti-goat secondary antibody (cat. no. BA-5000; Vector Laboratories, Newark, CA) and a polymer detection system (DS9800; Novocastra Bond Polymer Refine Detection; Leica Biosystems, Buffalo Grove, IL) were then applied to the tissues.A rabbit anti-goat secondary antibody (cat. no. BA-5000; Vector Laboratories, Newark, CA) and a polymer detection system (DS9800; Novocastra Bond Polymer Refine Detection; Leica Biosystems, Buffalo Grove, IL) were then applied to the tissues.

    Article Title: Chlamydia muridarum Infection Impacts Murine Models of Intestinal Inflammation and Cancer
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems, Deer Park, IL).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems, Deer Park, IL).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:2000.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Assessing the Biosecurity Risk of Footwear as a Fomite for Transmission of Adventitious Infectious Agents to Mice
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (catalog no. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra bond polymer refine detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (catalog no. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra bond polymer refine detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Assessing the Biosecurity Risk of Footwear as a Fomite for Transmission of Adventitious Infectious Agents to Mice
    Article Snippet: Briefly, formalin-fixed paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Chlamydia muridarum infection causes bronchointerstitial pneumonia in NOD.Cg- Prkdc scid Il2rg tm1Wjl /SzJ (NSG) mice
    Article Snippet: Formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, Colorado) was applied at a dilution of 1:2000.. A rabbit antigoat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, California) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit antigoat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, California) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Chlamydia muridarum Causes Persistent Subclinical Infection and Elicits Innate and Adaptive Immune Responses in C57BL/6J, BALB/cJ and J:ARC(S) Mice Following Exposure to Shedding Mice
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Staining:

    Article Title: Chlamydia muridarum Infection Impacts Murine Models of Intestinal Inflammation and Cancer
    Article Snippet: 1 Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX; Leica Biosystems, Buffalo Grove, IL).1 Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX; Leica Biosystems, Buffalo Grove, IL).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054; Novus Biologicals, Centennial, CO) was applied at a dilution of 1:2,000.. A rabbit anti-goat secondary antibody (cat. no. BA-5000; Vector Laboratories, Newark, CA) and a polymer detection system (DS9800; Novocastra Bond Polymer Refine Detection; Leica Biosystems, Buffalo Grove, IL) were then applied to the tissues.A rabbit anti-goat secondary antibody (cat. no. BA-5000; Vector Laboratories, Newark, CA) and a polymer detection system (DS9800; Novocastra Bond Polymer Refine Detection; Leica Biosystems, Buffalo Grove, IL) were then applied to the tissues.

    Article Title: Chlamydia muridarum Infection Impacts Murine Models of Intestinal Inflammation and Cancer
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems, Deer Park, IL).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems, Deer Park, IL).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:2000.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Assessing the Biosecurity Risk of Footwear as a Fomite for Transmission of Adventitious Infectious Agents to Mice
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (catalog no. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra bond polymer refine detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (catalog no. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra bond polymer refine detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Assessing the Biosecurity Risk of Footwear as a Fomite for Transmission of Adventitious Infectious Agents to Mice
    Article Snippet: Briefly, formalin-fixed paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Chlamydia muridarum infection causes bronchointerstitial pneumonia in NOD.Cg- Prkdc scid Il2rg tm1Wjl /SzJ (NSG) mice
    Article Snippet: Formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, Colorado) was applied at a dilution of 1:2000.. A rabbit antigoat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, California) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit antigoat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, California) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Chlamydia muridarum Causes Persistent Subclinical Infection and Elicits Innate and Adaptive Immune Responses in C57BL/6J, BALB/cJ and J:ARC(S) Mice Following Exposure to Shedding Mice
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Immunohistochemical staining:

    Article Title: Chlamydia muridarum Infection Impacts Murine Models of Intestinal Inflammation and Cancer
    Article Snippet: 1 Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX; Leica Biosystems, Buffalo Grove, IL).1 Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX; Leica Biosystems, Buffalo Grove, IL).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054; Novus Biologicals, Centennial, CO) was applied at a dilution of 1:2,000.. A rabbit anti-goat secondary antibody (cat. no. BA-5000; Vector Laboratories, Newark, CA) and a polymer detection system (DS9800; Novocastra Bond Polymer Refine Detection; Leica Biosystems, Buffalo Grove, IL) were then applied to the tissues.A rabbit anti-goat secondary antibody (cat. no. BA-5000; Vector Laboratories, Newark, CA) and a polymer detection system (DS9800; Novocastra Bond Polymer Refine Detection; Leica Biosystems, Buffalo Grove, IL) were then applied to the tissues.

    Article Title: Chlamydia muridarum Infection Impacts Murine Models of Intestinal Inflammation and Cancer
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems, Deer Park, IL).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems, Deer Park, IL).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:2000.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Assessing the Biosecurity Risk of Footwear as a Fomite for Transmission of Adventitious Infectious Agents to Mice
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (catalog no. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra bond polymer refine detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (catalog no. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra bond polymer refine detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Assessing the Biosecurity Risk of Footwear as a Fomite for Transmission of Adventitious Infectious Agents to Mice
    Article Snippet: Briefly, formalin-fixed paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Chlamydia muridarum infection causes bronchointerstitial pneumonia in NOD.Cg- Prkdc scid Il2rg tm1Wjl /SzJ (NSG) mice
    Article Snippet: Formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, Colorado) was applied at a dilution of 1:2000.. A rabbit antigoat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, California) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit antigoat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, California) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Chlamydia muridarum Causes Persistent Subclinical Infection and Elicits Innate and Adaptive Immune Responses in C57BL/6J, BALB/cJ and J:ARC(S) Mice Following Exposure to Shedding Mice
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Clinical Proteomics:

    Article Title: Chlamydia muridarum Infection Impacts Murine Models of Intestinal Inflammation and Cancer
    Article Snippet: 1 Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX; Leica Biosystems, Buffalo Grove, IL).1 Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX; Leica Biosystems, Buffalo Grove, IL).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054; Novus Biologicals, Centennial, CO) was applied at a dilution of 1:2,000.. A rabbit anti-goat secondary antibody (cat. no. BA-5000; Vector Laboratories, Newark, CA) and a polymer detection system (DS9800; Novocastra Bond Polymer Refine Detection; Leica Biosystems, Buffalo Grove, IL) were then applied to the tissues.A rabbit anti-goat secondary antibody (cat. no. BA-5000; Vector Laboratories, Newark, CA) and a polymer detection system (DS9800; Novocastra Bond Polymer Refine Detection; Leica Biosystems, Buffalo Grove, IL) were then applied to the tissues.

    Article Title: Chlamydia muridarum Infection Impacts Murine Models of Intestinal Inflammation and Cancer
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems, Deer Park, IL).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems, Deer Park, IL).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:2000.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Assessing the Biosecurity Risk of Footwear as a Fomite for Transmission of Adventitious Infectious Agents to Mice
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (catalog no. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra bond polymer refine detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (catalog no. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra bond polymer refine detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Assessing the Biosecurity Risk of Footwear as a Fomite for Transmission of Adventitious Infectious Agents to Mice
    Article Snippet: Briefly, formalin-fixed paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Chlamydia muridarum infection causes bronchointerstitial pneumonia in NOD.Cg- Prkdc scid Il2rg tm1Wjl /SzJ (NSG) mice
    Article Snippet: Formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, Colorado) was applied at a dilution of 1:2000.. A rabbit antigoat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, California) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit antigoat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, California) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.

    Article Title: Chlamydia muridarum Causes Persistent Subclinical Infection and Elicits Innate and Adaptive Immune Responses in C57BL/6J, BALB/cJ and J:ARC(S) Mice Following Exposure to Shedding Mice
    Article Snippet: Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).Briefly, formalin-fixed, paraffin-embedded sections were stained using an automated staining platform (Leica Bond RX, Leica Biosystems).. Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0, the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500.. A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.A rabbit anti-goat secondary antibody (Cat. No. BA-5000, Vector Laboratories, Burlingame, CA) and a polymer detection system (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied to the tissues.



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